实验目的:
多样品组织研磨机研磨导兔子腿骨,提取DNA。
实验地点:
首都医科大学附属友谊医院
实验材料和器具
样品:兔子腿骨
仪器:TISSUELYSER研磨机(上海净信,TISSUELYSER-24)
(上海净信,TISSUELYSER-24)
配件:不锈钢研磨罐2个(上海净信),
研磨珠(上海净信)
研磨条件:冷冻干磨
实验步骤
1. 将净信研磨罐置于液氮预冷备用
2.将兔子腿骨放在液氮中几分钟,然后剪成小块备用。
3.将小段的腿骨放在研磨罐里,盖好盖子。
4.将装好腿骨的研磨罐从液氮中取出装入研磨仪,拧紧旋钮。
4.设定好参数,启动仪器至研磨程序结束后再次预冷模块重复程序几次。
(样品实验前后对比)
Previous:关于菠萝果实采用全自动快速研磨仪研磨提取脂肪酸的一种方法-上海净信
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