樟树是一种用途很广的树种,在我国资源非常丰富,药用价值和绿化价值非常高。主要分布于我国南方各省,尤以福建台湾为最多。具有祛风,除湿,解毒,杀虫之功效。主治风湿痹痛,胃痛,水火烫伤,疮疡肿毒,慢性下肢溃疡,疥癣,皮肤瘙痒等。
近年来,国内外学者在樟树的化学成分和生物活性方面做了一些研究。研究发现樟树含有化学成分主要包括挥发性油、生物碱类、黄酮类、有机酸等。并且发现樟树的茎、叶、根樟树籽等各部分提取物具有一定的生物活性,主要涉及抑菌、驱虫、抗氧化、消炎等方面。
然而,这些方面的研究还不够系统,有必要对樟树叶的化学成分和生物活性进行更系统全面的研究。
下面内容讲述广东省林科院科研团队研磨樟树叶片前处理的方法步骤:
一、实验仪器多样品组织研磨仪Tissuelyser-48L(下图所示)
二、上海净信®的Tissuelyser®组织研磨仪(专利产品,仿冒必究!ZL2019301641779)是一种特殊的、快速的、高效率的、多试管的一致系统。它能将任何来源(包括土壤、植物和动物的组织/器官、细菌、酵母、真菌、孢子、古生物标本等)的原始DNA、RNA和蛋白质进行提取和纯化。Tissuelyser-48L采用垂直振荡方式,研磨更充分,1分钟内完成2×24、2×48、4×96个样品的研磨;同一组织样本设定相同程序,获得相同的研磨效果;内置程序控制器,可对研磨时间、转子的振动频率等参数进行设置;具备安全罩及安全锁;无交叉污染且低噪音。
实验步骤:
将樟树叶片剪小段后放入若干ML研磨罐中,加入特定规格的研磨介质,然后把研磨罐放入液氮众泡制几分钟,泡制至超低温后放进上海净信多样品研磨仪内,设置好研磨仪工作时间和频率,最后启动设备。
实验结果:
Previous:全自动组织研磨仪|研磨牙齿从牙齿中提取DNA
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